1 00:00:03,909 --> 00:00:02,070 thank you committee for the opportunity 2 00:00:05,190 --> 00:00:03,919 my name is rose i'm a secondary mpp 3 00:00:07,749 --> 00:00:05,200 fellow at parkinson university in new 4 00:00:10,629 --> 00:00:07,759 jersey usa so the main hypothesis of my 5 00:00:12,230 --> 00:00:10,639 project is a short 8 to 15 amino acid 6 00:00:14,070 --> 00:00:12,240 long peptide because the spontaneously 7 00:00:15,669 --> 00:00:14,080 formed on early earth 8 00:00:18,390 --> 00:00:15,679 that could have used the minerals in its 9 00:00:19,590 --> 00:00:18,400 surrounding to form a a short 10 00:00:22,870 --> 00:00:19,600 electron transferring or catalytic 11 00:00:24,550 --> 00:00:22,880 protein as shown in um figure one a 12 00:00:26,070 --> 00:00:24,560 great example of a catalytic protein 13 00:00:27,670 --> 00:00:26,080 includes hydrothen that's shown in 14 00:00:29,269 --> 00:00:27,680 figure two so what we wanted to do was 15 00:00:32,389 --> 00:00:29,279 really mimic the active site shown in 16 00:00:34,709 --> 00:00:32,399 the red circle and model a short peptide 17 00:00:37,190 --> 00:00:34,719 that could coordinate these metals and 18 00:00:39,510 --> 00:00:37,200 function as an s hydroxides right 19 00:00:40,389 --> 00:00:39,520 so uh and then what we're trying to do 20 00:00:42,069 --> 00:00:40,399 right now is the structural 21 00:00:44,229 --> 00:00:42,079 characterization uh figure three is 22 00:00:45,750 --> 00:00:44,239 showing if it is showing distinct two 23 00:00:48,069 --> 00:00:45,760 distinct species 24 00:00:50,549 --> 00:00:48,079 figure four epr of both reduce and 25 00:00:53,430 --> 00:00:50,559 oxidize form of our enzyme and figure 26 00:00:55,270 --> 00:00:53,440 five is a gas chromatography which shows 27 00:00:56,389 --> 00:00:55,280 the hydrogen production as compared to 28 00:00:57,750 --> 00:00:56,399 the control 29 00:01:00,150 --> 00:00:57,760 with that thank you and please don't